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The Expresso®BiotinCloning&ExpressionSystemsarenowavailablefromourbusinesscollaboratorLucigenCorporation. ThesesystemsofferyouthequickestroutetobiotinylatedproteinbycombiningtheAviTag™andEpressioneering™technologies.
LucigenhaspartneredwithAvidity,LLCtoleveragethepoweroftheAviTag™minimalbiotinylationmotifandLucigen"sSUMOsolubilityfusiontagtosolvethemostdifficultproblemsinproteinexpressionandpurification.TheincludedBiotinXCell™competentcellswillefficientlybiotinylateproteinbearingtheAviTagmotifinvivo,meaningyoucanskiptheinvitroandchemicalmethodsotherwiserequiredtoobtainbiotinylatedtargetprotein.TheSUMOtagwillproducelargeamountsofsolubleproteinthatcanbepreciselycleavedwithitscognateprotease.SinglecolumnpurificationofAviTagproteinsiscompletedinafewhoursusingthebiotin-streptavidininteraction,andoncolumncleavageoftheAviTag-SUMOfusionfollowedbynickelcolumnchromatographyoftheHIStaggedproteaseyields>95%pureprotein.